Authors: Wenzhen Li, Yifan Wang, Xinbo Zhou, Xiaohong Pan, Junhong Lü, Hongliu Sun, Zeping Xie, Shayan Chen, Xue Gao

Journal: Journal of Ginseng Research (2021)

Institution: Binzhou Medical University

Research Areas: Cancer research

Cell Lines: HepG2, H22 (Human hepatocarcinoma cells; mouse hepatocellular carcinoma (HCC) cell line)

Summary: 20(S)-protopanaxadiol (20(S)-PPD), one of the main active metabolites of ginseng, performs a broad spectrum of anti-tumor effects. The aims are to search out new strategies to enhance the anti-tumor effects of natural products, including 20(S)-PPD. Here, the effects of fasting combined with 20(S)-PPD on hepatocellular carcinoma growth, apoptosis, migration, invasion, and cell cycle were explored. Results showed that fasting significantly sensitized HCC cells to 20(S)-PPD in vivo and in vitro. These data indicated that dietary restriction could be one of the potential strategies of Chinese medicine or its active metabolites against hepatocellular carcinoma. In addition, HoloMonitor M4 is used to study cell proliferation and single-cell tracking.

Keywords: HoloMonitor M4, cell proliferation, single cell track, 20(S)-protopanaxadiolfasting-mimickingintermittent fasting, hepatocellular carcinoma