Authors: Livia P. Mendes, Nina Filipczak, Xiang Li, Ed Luther, Jacob S. Brenner, Marco Zamora, Oscar A. Marcos-Contreras, Jacob W. Myerson, Kandace Gollomp, Kobra Rostamizadeh, Vladimir P. Torchilin

Journal: mAbs (2020)

Institution: Northeastern University

Research Areas: Cell Research

Cell Lines: HL-60 (Human leukemia cell line)

Summary: Neutrophil extracellular traps (NETs) are a defense mechanism against pathogens, such as bacteria, they have also been implicated as an important part of a wide range of pathologies, such as protecting tumor cells from T lymphocytes and natural killer cells; involved in acute thrombotic complications in cardiovascular and autoimmune disease, and most recently it was shown that NETs may contribute to organ damage and mortality in COVID-19. Thus, it is important to detect and remove NETs when their pathological role becomes dominant. In this publication, L. P. Mendes with colleagues have developed a method to visualize and detect NET-affected areas using 2C5 antibody modified nanocarriers. Authors have employed HoloMonitor M4 to study HL-60 cell morphology after treatment with differentiation agents.

Keywords: HoloMonitor M4, cell morphology, neutrophil extracellular traps, monoclonal antibody, active targeting2C5, microfluidic study