Northeastern University – Holographic Imaging Cytometry Program of Excellence

The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis. The program covers several activities including:

  • Establishing a network of collaborators utilizing Holographic Imaging Cytometry.
  • Developing novel applications supporting research interests of the Northeastern faculty and students.
  • Presenting results at scientific meetings.
  • Organizing an annual technology symposium and hands-on laboratory courses.

Achievements

In May 2017, a summary of the scientific achievements was published in a special issue of Cytometry part A, dedicated to holographic microscopy and quantitative phase imaging.

Read the summary here and view the associated CYTO 2017 presentation here.

Poster Session

Poster session at AACR Annual Meeting 2017. View poster presented by Ed Luther, Supervisor Core Imaging and Cytometry Facility at Northeastern University here.

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The expansion of our existing collaboration with PHI is the result of productive utilization of HoloMonitor technology for non-invasive real-time cell cycle analysis in living cells. We are now developing state-of-the art applications multiplexing cell cycle and cell motility.

Ed Luther

Northeastern University

Peer Reviewed Articles and Book Chapters

Targeted Delivery of Combination Therapeutics Using Monoclonal Antibody 2C5-Modified Immunoliposomes for Cancer Therapy

Authors: Radhika Narayanaswamy et al.

Journal: Pharmaceutical Research (2021)

Research Areas: Drug research

Cell Lines: MDA-MD-231

Keywords: HoloMonitor M4, cell Proliferation and cell Morphology, cancer therapy

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Monoclonal antibody 2C5 specifically targets neutrophil extracellular traps

Authors: Livia P. Mendes et al.

Journal: mAbs (2020)

Research Areas: Cell Research

Cell Lines: HL-60

Keywords: HoloMonitor M4, cell morphology, neutrophil extracellular traps, monoclonal antibody, active targeting2C5, microfluidic study

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Advancing methods for the analysis of glioblastoma cell motion using quantitative time lapse holographic imaging and cellular tomography

Authors: Ed Luther et al.

Journal: SPIE BiOS (2019)

Research Areas: Cancer research

Cell Lines: Glioblastoma U87

Keywords: HoloMonitor M4, Cell movement, Holography, Tomography, Refractive index, Visualization, Motion analysis

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Applications of quantitative time lapse holographic imaging to the development of complex pharmaceutical nano formulations

Authors: Ed Luther et al.

Journal: SPIE BiOS Conference Proceedings (2018)

Research Areas: Method development

Cell Lines: Tumor micro-environment

Keywords: HoloMonitor M4, Holography, Image segmentation, Standards development, Tumors, Image processing, Imaging systems, 3D image processing, Etching, Holograms

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Applications of Label-free, Quantitative Phase Holographic Imaging Cytometry to the Development of Multi-specific Nanoscale Pharmaceutical Formulations

Authors: Ed Luther et al.

Journal: Cytometry Part A 2017 (2017)

Research Areas: Pharmacology

Cell Lines: HeLa, L929

Keywords: Review, HoloMonitor M4, Holographic imaging, Label-free, Time-lapse, 4-D imaging

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Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of a p-glycoprotein Inhibitor and Paclitaxel in a Liposomal Platform

Authors: Yilin Zhang et al.

Journal: Molecular Cancer Therapeutics (2016)

Research Areas: Cancer research

Cell Lines: SKOV 3 TR cells

Keywords: HoloMonitor M4, Cell cycle arrest, Apoptosis, Ovarian cancer

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Mixed Nanosized Polymeric Micelles as Promoter of Doxorubicin and Mirna-34a Co-delivery Triggered by Dual Stimuli in Tumor Tissue

Authors: Giuseppina Salzano et al.

Journal: Small (2016)

Research Areas: Drug research, Cancer research

Cell Lines: HT1080

Keywords: HoloMonitor M4, Cell motility, Cell morphology

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Enhanced Cytotoxicity of Folic Acid-targeted Liposomes Co-loaded With C6 Ceramide and Doxorubicin: in Vitro Evaluation on HeLa, A2780-ADR and H69-AR Cells

Authors: Shravan Kumar Sriraman et al.

Journal: Molecular pharmaceutics (2015)

Research Areas: Cancer research, Drug research

Cell Lines: HeLa

Keywords: HoloMonitor M4, Liposomes, Combination, Drug Therapy, Nanoparticles, Folic Acid, Cancer, Drug Resistance, Receptor Targeting, Apoptosis, Cell Cycle Arrest, Nano medicine, Spheroids, drug development

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Macrophage Polarization and the Effect of Microrna-155 Administered in Water-in-oil-in-water Multiple Emulsion Formulations

Authors: Adwait Oka et al.

Journal: Journal of Clinical & Cellular Immunology (2015)

Research Areas: Cancer research, Immunology

Cell Lines: J774A, 1, SKOV3

Keywords: Holomonitor M4, cell Morphology, cell motility, tumor-associated macrophages, Macrophage polarization, MicroRNA-155, Water-in-oil-in-water multiple emulsions

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Conference Papers and Posters

Characterization of a green, temperature activated nano formulation that drives the mechanism of doxorubicin toxicity from apoptosis to ferroptosis

Authors: Farzana Parveen et al.

Journal: AACR Annual Meeting (2022)

Research Areas: Cancer research, Biotechnology

Cell Lines: B16F10, U87-MR, MDA-MB-231, MCF-7

Keywords: temperature-activated nano formulation, TAN, doxorubicin, ferroptosis

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Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holographic imaging

Authors: Ed Luther et al.

Journal: AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)

Research Areas: Cancer research

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Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture Models

Authors: Ed Luther et al.

Journal: AACR Annual Meeting (2017)

Research Areas: Drug research

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A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov Test

Authors: Ed Luther et al.

Journal: 3rd Conference on Impedance-Based Cellular Assays (2016)

Research Areas: Biotechnology

Keywords: Drug delivery

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Four dimensional quantitative label-free holographic imaging of the cell cycle in tumor cell lines

Authors: Ed Luther et al.

Journal: AACR Annual Meeting (2015)

Research Areas: Cancer research

Keywords: Poster, digital holography, cell cycle

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Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imaging

Authors: Ed Luther et al.

Journal: AACR Annual Meeting (2015)

Research Areas: Cancer research

Keywords: Poster, tumor microenvironment, macrophage, digital holography

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Featured Applications

Chemoresistance

Macrophage Polarization

Macrophage Response to Polarization

Location

Department of Pharmaceutical Sciences
School of Pharmacy
Bouvé College of Health Sciences
Northeastern University
140 The Fenway, Room 228/229
60 Huntington Avenue
Boston, Massachusetts 02115

The Partnerships

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Lund University Cancer Centre

The early HoloMonitor development began at Lund University. The relationship between Lund University and PHI has since evolved into partnerships with several research groups.
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Uppsala University

Supported by PHI, scientists at Uppsala University apply state of the art artificial intelligence on its very source of inspiration — cell biology.
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UCSF

University of California in San Francisco, Gladstone Institutes and PHI jointly operate San Francisco’s Holographic Imaging Cytometry Center of Excellence.
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National Institute on Aging

In pursuit of making novel research tools available to National Institute on Aging and the medical research community in general, NIA and PHI are collaborating to develop a fluorescence module to PHI’s HoloMonitor.
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Gladstone Institutes

Gladstone Institutes, UCSF and PHI jointly operate San Francisco’s Holographic Imaging Cytometry Center of Excellence.
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Huntsman Cancer Institute

Supported by PHI, Dr. Robert Judson-Torres and his team at Huntsman Cancer Institute aim to develop methods to quantify the phenomena known as epithelial-mesenchymal transition (EMT).
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Malmö University

PHI and Malmö University have a long-standing collaboration, dating back to 2008. The collaboration has resulted in several peer reviewed publications and a doctoral thesis.